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Kelsey A. Johnson James Sutherland-Smith Trisha J. Oura Amy F. Sato Bruce Barton 《Veterinary radiology & ultrasound》2023,64(1):86-94
Evaluation of brain disease in veterinary patients uses a wide variety of MRI sequences. A shortened protocol that maintains consistency of interpretation would reduce radiologist reporting time, patient anesthetic time, and client cost. The aims of this retrospective, methods comparison, observer agreement study were to evaluate whether abbreviated MRI protocols alter differential diagnoses and recommendations compared to our institution's standard protocol; evaluate interobserver agreement on standard brain MRIs; and assess whether differential diagnoses change after postcontrast images. Normal and pathologic canine and feline brain MRIs were retrieved from hospital archives. Three protocols were created from each: a 5-sequence noncontrast enhanced Fast Brain Protocol 1 (FBP1); a 6-sequence contrast-enhanced Fast Brain Protocol 2 (FBP2); and an 11-sequence standard brain protocol (SBP). Three blinded veterinary radiologists interpreted FBP images for 98 cases (1 reader/case) and SBP images for 20 cases (3 readers/case). A fourth observer compared these interpretations to the original MRI reports (OMR). Overall agreement between FBPs and OMR was good (k = 0.75) and comparable to interobserver agreement for multiple reviews of SBP cases. Postcontrast images substantially altered conclusions in 17/97 cases (17.5%), as well as improved interobserver agreement compared to noncontrast studies. The conclusions reached with shortened brain protocols were comparable to those of a full brain study. The findings supported the use of a 6-sequence brain MRI protocol (sagittal T2-weighted [T2w] TSE; transverse T2w turbo spin echo fluid-attenuated inversion recovery, T2*-weighted gradient recalled echo, T1-weighted spin echo, and diffusion weighted imaging/apparent diffusion coefficient; and postcontrast transverse T1-weighted spin echo) for dogs and cats with suspected intracranial disease. 相似文献
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Matt J. Griffin Stephen R. Reichley Wes A. Baumgartner Suja Aarattuthodiyil Cynthia Ware James M. Steadman Marsha Lewis Patricia S. Gaunt Lester H. Khoo David J. Wise 《Journal of the World Aquaculture Society》2019,50(2):420-432
There is a trend toward the increased incidence and prevalence of Edwardsiella piscicida septicemia in US catfish aquaculture, particularly in channel ♀, Ictalurus punctatus, × blue ♂, I. furcatus, hybrid catfish. From 2013 to 2017, a total of 3242 disease case submissions were made to the Aquatic Research and Diagnostic Laboratory (ARDL) at the Thad Cochran National Warmwater Aquaculture Center in Stoneville, MS. Of these, 1400 (43.2%) were hybrids. E. piscicida was suspected in 138 (4.3%) of cases, the majority of which (89.1%) were from hybrid catfish. A molecular survey of these isolates confirmed the majority (92.0%) to be E. piscicida. Furthermore, cases of E. piscicida from hybrids submitted to the ARDL and the Aquatic Diagnostic Laboratory of the Mississippi State University College of Veterinary Medicine in Starkville, MS, were documented for gross lesions and histological analysis. Grossly, E. piscicida presents with small dermal ulcerations, a raised fluid‐filled cranial midline lesion that is frequently ulcerated, hemorrhage in the gills, exophthalmia, and abdominal distension. Internally, lesions include splenomegaly, straw‐colored ascites, renomegaly, and occasionally hemorrhagic intestines. Histopathological examination is in agreement with gross observations, and infected fish repeatedly demonstrate a mononuclear meningoencephalitis, hemorrhagic branchitis, splenitis, ulcerative dermatitis, granulomatous interstitial nephritis, and hepatitis coupled with a hemorrhagic enteritis. 相似文献
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Lampreys have a complex life cycle which includes a multi‐year infaunal larval stage (ammocoete). Gut content analysis has generally identified detritus (i.e., unidentifiable organic matter) as the major dietary component to ammocoetes, though algae can also be important. However, gut content preserves only a snapshot of the animal's diet and does not reflect assimilated material. In order to better characterise the nutritional sources supporting ammocoete growth, we analysed ammocoete body tissue and potential dietary sources at two streams using natural Δ14C and δ15N to estimate time‐integrated nutritional support. Bayesian isotope mixing models revealed differences in the importance of sources supporting ammocoetes between sites. Ammocoetes from a stream in a mixed land usage area (~50% agriculture, ~40% forest and ~10% developed) were primarily supported (mean: ~50%) by fresh terrestrial organic matter but were also supported by substantial contributions (mean: ~30%) by aged organic matter (AOM) and autochthonous material (algae; mean ~20%). In a predominantly forested (~90%) headwater stream, different modelling scenarios (uninformed or informed priors) suggested that algal support of ammocoete nutrition ranged from 7% to 45%. However, the model relying on informed priors developed from gut content analysis produced the low estimates, suggesting these were more reliable. When algae were a minor component of the nutrition at the forested site, ammocoetes were highly dependent on AOM (83 ± 26%; mean ± SD). Based on these findings, ammocoete growth and development are predicted to be strongly influenced by both land use and the availability of allochthonous and autochthonous materials of varying ages within streams. 相似文献
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